KMID : 1004520210370020088
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Journal of Dental Rehabilitation and Applied Science 2021 Volume.37 No. 2 p.88 ~ p.94
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Anti-inflammatory effect of non-thermal atmospheric pressure plasma for periodontitis treatment: in vitro pilot study
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Park You-li
Kim Hyun-Joo Lee Ju-Youn Jung Sung-Hee Kwon Eun-Young Joo Ji-Young
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Abstract
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Purpose: The purpose of this study was to evaluate the anti-inflammatory effects of non-thermal atmospheric pressure plasma (NTP) on human gingival fibroblasts (HGFs) for clinical application of periodontal treatment. Materials and
Methods: HGFs were treated with Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS). Customized NTP device was developed for periodontal in vitro study. Cell viability was evaluated with cell counting kit-8. The levels of inflammatory cytokines, including interleukin (IL)-8 and 6, were determined by enzyme-linked immunosorbent assay.
Results: When NTP was applied, the cell viability did not change significantly, and there was no difference for 6 h and 24h. When Pg LPS was treated to HGFs, the secretion of IL-8 and IL-6 was increased compared to the control group. But when the NTP was applied, the secretion of them was significantly decreased.
Conclusion: NTP did not affect cell viability of HGFs. And it inhibited the LPS-induced production of IL-8 and IL-6.
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KEYWORD
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periodontitis, non-thermal atmospheric pressure plasma, pilot study, anti-inflammatory, human gingival fibroblast
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